Streptococcus pyogenes Cas9

URI: https://gptkb.org/entity/Streptococcus_pyogenes_Cas9
GPTKB entity
Statements (49)
Predicate Object
gptkbp:instanceOf gptkb:protein
gptkbp:alsoKnownAs gptkb:SpCas9
gptkbp:cleavage double-stranded DNA
gptkbp:component gptkb:CRISPR-Cas9_system
gptkbp:discoveredBy gptkb:Emmanuelle_Charpentier
gptkb:Jennifer_Doudna
gptkbp:discoveredIn 2012
gptkbp:domain gptkb:PAM-interacting_domain
gptkb:HNH
gptkb:RuvC
gptkbp:foundIn gptkb:Streptococcus_pyogenes
gptkbp:function gptkb:endonuclease
genome editing
gptkbp:gene cas9
gptkbp:molecularWeight ~160 kDa
gptkbp:recognizesPAM gptkb:NGG
gptkbp:requires gptkb:guide_RNA
gptkbp:structure bilobed
gptkbp:usedFor genome editing in eukaryotes
genome editing in prokaryotes
gptkbp:usedIn gptkb:gene_therapy
gptkb:biotechnology
gptkb:gene_drive
gptkb:CRISPR_interference
agriculture
biomedical research
drug discovery
personalized medicine
synthetic biology
diagnostics
genome engineering
base editing
prime editing
disease modeling
functional genomics
cell line engineering
crop improvement
epigenome editing
gene activation
gene knockout
gene repression
high-throughput screening
transcriptional regulation
animal model generation
antiviral research
bacterial immunity
gptkbp:bfsParent gptkb:catalytically_dead_Cas9
gptkbp:bfsLayer 8
https://www.w3.org/2000/01/rdf-schema#label Streptococcus pyogenes Cas9