Statements (49)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
gptkb:protein
|
| gptkbp:alsoKnownAs |
gptkb:SpCas9
|
| gptkbp:cleavage |
double-stranded DNA
|
| gptkbp:component |
gptkb:CRISPR-Cas9_system
|
| gptkbp:discoveredBy |
gptkb:Emmanuelle_Charpentier
gptkb:Jennifer_Doudna |
| gptkbp:discoveredIn |
2012
|
| gptkbp:domain |
gptkb:PAM-interacting_domain
gptkb:HNH gptkb:RuvC |
| gptkbp:foundIn |
gptkb:Streptococcus_pyogenes
|
| gptkbp:function |
genome editing
endonuclease |
| gptkbp:gene |
cas9
|
| https://www.w3.org/2000/01/rdf-schema#label |
Streptococcus pyogenes Cas9
|
| gptkbp:molecularWeight |
~160 kDa
|
| gptkbp:recognizesPAM |
gptkb:NGG
|
| gptkbp:requires |
gptkb:guide_RNA
|
| gptkbp:structure |
bilobed
|
| gptkbp:usedFor |
genome editing in eukaryotes
genome editing in prokaryotes |
| gptkbp:usedIn |
gptkb:biotechnology
gptkb:gene_drive gptkb:CRISPR_interference agriculture biomedical research drug discovery personalized medicine synthetic biology diagnostics genome engineering base editing gene therapy prime editing disease modeling functional genomics cell line engineering crop improvement epigenome editing gene activation gene knockout gene repression high-throughput screening transcriptional regulation animal model generation antiviral research bacterial immunity |
| gptkbp:bfsParent |
gptkb:catalytically_dead_Cas9
|
| gptkbp:bfsLayer |
7
|