Statements (51)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
Molecular biology technique
|
| gptkbp:alsoKnownAs |
gptkb:real-time_PCR
qPCR |
| gptkbp:appliesTo |
forensic science
environmental monitoring food safety testing clinical diagnostics |
| gptkbp:basedOn |
gptkb:polymerase_chain_reaction
|
| gptkbp:canBe |
multiplex
real-time analysis endpoint analysis SYBR Green-based TaqMan probe-based absolute quantification method comparative Ct method relative quantification method relative standard curve method singleplex standard curve method |
| gptkbp:detects |
gptkb:gene
cDNA RNA (with reverse transcription) amplified DNA in real time |
| gptkbp:developedBy |
1990s
|
| gptkbp:enables |
absolute quantification
high-throughput analysis relative quantification |
| https://www.w3.org/2000/01/rdf-schema#label |
Quantitative PCR
|
| gptkbp:measures |
cycle threshold (Ct) value
|
| gptkbp:monitors |
amplification during each cycle
|
| gptkbp:provides |
qualitative data
quantitative data |
| gptkbp:relatedTo |
gptkb:conventional_PCR
gptkb:digital_PCR |
| gptkbp:requires |
nucleotides
DNA polymerase primers template DNA thermal cycler with fluorescence detection calibration curve for absolute quantification careful experimental design |
| gptkbp:sensitivity |
contamination
primer-dimer formation PCR inhibitors |
| gptkbp:usedFor |
gene expression analysis
detection of pathogens quantification of nucleic acids |
| gptkbp:uses |
fluorescent dyes
sequence-specific DNA probes |
| gptkbp:bfsParent |
gptkb:Droplet_Digital_PCR
|
| gptkbp:bfsLayer |
8
|