Enzyme-Linked Immunosorbent Assay
GPTKB entity
Statements (51)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
immunoassay
|
| gptkbp:abbreviation |
gptkb:ELISA
|
| gptkbp:advantage |
high sensitivity
high specificity quantitative results |
| gptkbp:application |
HIV testing
pregnancy testing cytokine measurement food allergen detection hormone quantification infectious disease diagnosis |
| gptkbp:detects |
enzyme-linked detection
colorimetric detection |
| gptkbp:developedBy |
1970s
|
| https://www.w3.org/2000/01/rdf-schema#label |
Enzyme-Linked Immunosorbent Assay
|
| gptkbp:inventedBy |
gptkb:Eva_Engvall
gptkb:Peter_Perlmann |
| gptkbp:limitation |
false positives
cross-reactivity requires optimization |
| gptkbp:method |
plate-based assay
|
| gptkbp:regulates |
gptkb:CDC
gptkb:FDA |
| gptkbp:relatedTo |
gptkb:Western_blot
gptkb:immunofluorescence_assay gptkb:lateral_flow_assay radioimmunoassay |
| gptkbp:requires |
gptkb:monoclonal_antibody
substrate microtiter plate enzyme conjugate |
| gptkbp:step |
washing
detection coating blocking incubation signal measurement |
| gptkbp:type |
gptkb:plasma
serum cell lysate |
| gptkbp:usedFor |
detection of antibodies
detection of antigens quantification of proteins |
| gptkbp:usedIn |
research laboratories
clinical diagnostics |
| gptkbp:variant |
gptkb:competitive_ELISA
gptkb:direct_ELISA gptkb:indirect_ELISA gptkb:sandwich_ELISA |
| gptkbp:bfsParent |
gptkb:ELISA
|
| gptkbp:bfsLayer |
5
|