Statements (50)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
laboratory technique
|
| gptkbp:alsoKnownAs |
immunoblot
|
| gptkbp:alternativeTo |
gptkb:ELISA
mass spectrometry |
| gptkbp:canBe |
disease diagnosis
post-translational modification analysis protein-protein interaction studies protein purity assessment protein size determination |
| gptkbp:canQuantify |
protein expression levels
|
| gptkbp:detects |
gptkb:HIV_antibodies
colorimetric detection fluorescence detection Lyme disease antibodies chemiluminescence specific proteins |
| gptkbp:developedBy |
gptkb:W._Neal_Burnette
1979 |
| https://www.w3.org/2000/01/rdf-schema#label |
Western blot
|
| gptkbp:involves |
gel electrophoresis
antibody probing protein transfer to membrane |
| gptkbp:limitation |
time-consuming
requires specific antibodies semi-quantitative |
| gptkbp:membraneType |
gptkb:PVDF
nitrocellulose |
| gptkbp:namedAfter |
gptkb:Southern_blot
|
| gptkbp:publishedIn |
gptkb:Analytical_Biochemistry
|
| gptkbp:relatedTo |
gptkb:Northern_blot
gptkb:Southern_blot |
| gptkbp:requires |
imaging system
electrophoresis apparatus primary antibody secondary antibody transfer apparatus |
| gptkbp:step |
gptkb:SDS-PAGE
detection blocking transfer sample preparation antibody incubation |
| gptkbp:usedFor |
protein detection
protein quantification |
| gptkbp:usedIn |
biochemistry
molecular biology immunogenetics |
| gptkbp:bfsParent |
gptkb:Lyme_Disease
gptkb:ELISA |
| gptkbp:bfsLayer |
5
|