Western blot

GPTKB entity

Statements (56)
Predicate Object
gptkbp:instance_of gptkb:physicist
gptkbp:analysis protein expression levels
gptkbp:analyzes fluorescence
chemiluminescence
gptkbp:developed_by gptkb:W._Neal_Burnette
https://www.w3.org/2000/01/rdf-schema#label Western blot
gptkbp:involves gel electrophoresis
transfer to membrane
gptkbp:is_affected_by sample quality
gptkbp:is_analyzed_in biomarkers
gene expression
cell signaling pathways
protein purity
gptkbp:is_essential_for target proteins
gptkbp:is_often_associated_with immunoprecipitation
gptkbp:is_often_used_in molecular biology
biochemistry
cell biology
mass spectrometry
pharmaceutical development
protein detection
gptkbp:is_studied_in protein function
disease mechanisms
protein-protein interactions
protein localization
gptkbp:is_tested_for antibody specificity
gptkbp:is_used_for detecting specific proteins
results from other assays
gptkbp:is_used_in gptkb:Research_Institute
clinical diagnostics
gptkbp:monitors disease progression
gptkbp:performed_by laboratories
gptkbp:recognizes gptkb:monoclonal_antibody
post-translational modifications
antigens
mutant proteins
gptkbp:requires sample preparation
primary antibodies
secondary antibodies
gptkbp:sensor protein concentration
gptkbp:technique automated
costly
time-consuming
proteomics
quantitative or qualitative
protein analysis
protein research
standardized.
careful optimization
proper controls
gptkbp:type_of immunoblotting
gptkbp:uses gptkb:monoclonal_antibody
gptkbp:bfsParent gptkb:HIV-2
gptkb:Feline_Immunodeficiency_Virus
gptkb:Feline_immunodeficiency_virus
gptkbp:bfsLayer 4