Statements (23)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
immunoassay
|
| gptkbp:analyte |
gptkb:monoclonal_antibody
|
| gptkbp:detects |
antibodies in sample
|
| gptkbp:developedBy |
1970s
|
| https://www.w3.org/2000/01/rdf-schema#label |
indirect ELISA
|
| gptkbp:measures |
colorimetric change
|
| gptkbp:relatedTo |
gptkb:competitive_ELISA
gptkb:direct_ELISA gptkb:sandwich_ELISA |
| gptkbp:requires |
microtiter plate
washing steps blocking agent |
| gptkbp:step |
antigen coating
enzyme-substrate reaction primary antibody binding secondary antibody binding |
| gptkbp:usedFor |
diagnosis of infectious diseases
serological testing antibody detection |
| gptkbp:uses |
chromogenic substrate
enzyme-linked secondary antibody |
| gptkbp:bfsParent |
gptkb:ELISA
|
| gptkbp:bfsLayer |
5
|