Statements (51)
| Predicate | Object |
|---|---|
| gptkbp:instance_of |
gptkb:immunotherapy
|
| gptkbp:automated |
gptkb:true
|
| gptkbp:can_detect |
biomarkers
cytokines hormones antigens |
| gptkbp:developed_by |
gptkb:C._E._H._Harlow
|
| gptkbp:first_antibody |
capture antibody
|
| gptkbp:has_limitations |
time constraints
matrix effects sample volume cost of reagents cross-reactivity |
| https://www.w3.org/2000/01/rdf-schema#label |
sandwich ELISA
|
| gptkbp:involves |
incubation steps
washing steps |
| gptkbp:is_analyzed_in |
concentration of analytes
|
| gptkbp:is_often_used_in |
gptkb:research
clinical diagnostics |
| gptkbp:is_optimized_for |
temperature
antibody concentration buffer composition incubation time substrate choice |
| gptkbp:is_popular_in |
gptkb:biotechnology
gptkb:pharmaceuticals gptkb:academic_research clinical laboratories diagnostic companies |
| gptkbp:is_qualitative |
false
|
| gptkbp:is_quantitative |
gptkb:true
|
| gptkbp:is_recommended_by |
competitive ELISA
direct ELISA |
| gptkbp:is_used_in |
environmental monitoring
food safety testing pharmaceutical testing vaccine development |
| gptkbp:performed_in |
microtiter plates
|
| gptkbp:requires |
standard curve
two antibodies |
| gptkbp:second_antibody |
detection antibody
|
| gptkbp:sensitivity |
sample quality
antibody affinity antibody specificity |
| gptkbp:used_for |
detecting proteins
|
| gptkbp:uses |
chemiluminescent detection
colorimetric detection fluorescent detection enzyme-linked antibodies |
| gptkbp:bfsParent |
gptkb:ELISA
|
| gptkbp:bfsLayer |
5
|