Statements (38)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
immunoassay
|
| gptkbp:advantage |
high sensitivity
high specificity |
| gptkbp:detects |
gptkb:enzyme-linked_immunosorbent_assay
immune response chemiluminescent colorimetric fluorometric |
| https://www.w3.org/2000/01/rdf-schema#label |
sandwich ELISA
|
| gptkbp:inventedBy |
1970s
|
| gptkbp:notRecommendedFor |
small molecules
|
| gptkbp:quantitative |
yes
|
| gptkbp:relatedTo |
gptkb:competitive_ELISA
gptkb:direct_ELISA gptkb:indirect_ELISA |
| gptkbp:requires |
capture antibody
detection antibody enzyme-conjugated antibody two antibodies recognizing different epitopes |
| gptkbp:semiQuantitative |
yes
|
| gptkbp:step |
signal detection
blocking adding detection antibody adding enzyme-conjugated antibody adding sample coating with capture antibody substrate addition |
| gptkbp:type |
gptkb:plasma
serum cell lysate |
| gptkbp:usedFor |
protein quantification
antigen detection |
| gptkbp:usedIn |
research laboratories
food industry environmental monitoring clinical diagnostics |
| gptkbp:bfsParent |
gptkb:ELISA
|
| gptkbp:bfsLayer |
5
|