direct ELISA

GPTKB entity

Statements (23)
Predicate Object
gptkbp:instanceOf immunoassay
gptkbp:advantage faster than indirect ELISA
simple protocol
gptkbp:detects enzyme-linked antibody
https://www.w3.org/2000/01/rdf-schema#label direct ELISA
gptkbp:involves antigen immobilization
enzyme-conjugated primary antibody
gptkbp:limitation lower sensitivity
higher background noise
less flexible
gptkbp:relatedTo gptkb:competitive_ELISA
gptkb:indirect_ELISA
gptkb:sandwich_ELISA
gptkbp:step adding enzyme-linked antibody
adding substrate
coating plate with antigen
measuring signal
gptkbp:type chemiluminescent
colorimetric
fluorometric
gptkbp:usedFor detecting antigens
gptkbp:bfsParent gptkb:ELISA
gptkbp:bfsLayer 5