|
gptkbp:instanceOf
|
immunoassay
|
|
gptkbp:advantage
|
high sensitivity
high specificity
quantitative results
|
|
gptkbp:alternativeTo
|
radioimmunoassay
|
|
gptkbp:application
|
HIV testing
pregnancy testing
cytokine measurement
food allergen detection
hormone quantification
|
|
gptkbp:commonEnzymes
|
gptkb:alkaline_phosphatase
gptkb:horseradish_peroxidase
|
|
gptkbp:detects
|
enzyme-linked detection
|
|
gptkbp:developedBy
|
gptkb:Eva_Engvall
gptkb:Peter_Perlmann
1971
|
|
gptkbp:fullName
|
gptkb:Enzyme-Linked_Immunosorbent_Assay
|
|
https://www.w3.org/2000/01/rdf-schema#label
|
ELISA
|
|
gptkbp:limitation
|
time-consuming
cross-reactivity
requires multiple steps
|
|
gptkbp:method
|
plate-based assay
|
|
gptkbp:readBy
|
chemiluminescent
colorimetric
fluorometric
|
|
gptkbp:regulates
|
FDA (for clinical use)
|
|
gptkbp:relatedTo
|
gptkb:Western_blot
gptkb:immunofluorescence_assay
gptkb:lateral_flow_assay
|
|
gptkbp:requires
|
antibodies
substrate
microtiter plate
|
|
gptkbp:type
|
gptkb:plasma
gptkb:competitive_ELISA
gptkb:direct_ELISA
gptkb:indirect_ELISA
gptkb:sandwich_ELISA
urine
serum
cell lysates
|
|
gptkbp:usedFor
|
diagnosing diseases
detecting antibodies
detecting antigens
quantifying proteins
|
|
gptkbp:usedIn
|
research laboratories
food industry
clinical laboratories
veterinary diagnostics
|
|
gptkbp:bfsParent
|
gptkb:immunologist
|
|
gptkbp:bfsLayer
|
4
|