Statements (41)
| Predicate | Object |
|---|---|
| gptkbp:instance_of |
gptkb:immunotherapy
|
| gptkbp:benefits |
quantitative results
high sensitivity time-consuming high specificity requires specific reagents potential for cross-reactivity |
| gptkbp:components |
gptkb:Software_Solutions
gptkb:monoclonal_antibody substrate enzyme-linked antibody |
| gptkbp:developed_by |
gptkb:1971
gptkb:Peter_Perlmann |
| https://www.w3.org/2000/01/rdf-schema#label |
ELISA
|
| gptkbp:key_figures |
can be automated for high throughput
can test multiple samples simultaneously detection limit in picograms dynamic range of several orders of magnitude typical assay time of 2-4 hours |
| gptkbp:notable_applications |
clinical research
food safety testing diagnosing diseases detecting antibodies measuring hormone levels |
| gptkbp:protocol |
measuring absorbance
adding enzyme-linked antibody adding sample adding substrate blocking non-specific sites coating plate with antigen |
| gptkbp:technique |
Western blotting
flow cytometry immunofluorescence radioimmunoassay |
| gptkbp:type_of |
gptkb:sandwich_ELISA
competitive ELISA direct ELISA indirect ELISA |
| gptkbp:used_for |
detecting and quantifying proteins
|
| gptkbp:bfsParent |
gptkb:Oncology
|
| gptkbp:bfsLayer |
4
|