Statements (24)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
analytical technique
|
| gptkbp:alsoKnownAs |
gptkb:SEC-HPLC
gptkb:size_exclusion_high_performance_liquid_chromatography |
| gptkbp:application |
quality control
pharmaceutical analysis molecular weight determination |
| gptkbp:detects |
refractive index
light scattering UV absorbance |
| gptkbp:elutionOrder |
large molecules elute first
small molecules elute last |
| https://www.w3.org/2000/01/rdf-schema#label |
size exclusion HPLC
|
| gptkbp:mobilePhase |
aqueous buffer
organic solvent |
| gptkbp:principle |
molecules are separated based on hydrodynamic volume
|
| gptkbp:relatedTo |
gptkb:HPLC
gptkb:gel_filtration_chromatography gptkb:size_exclusion_chromatography |
| gptkbp:type |
packed with porous beads
|
| gptkbp:usedFor |
analysis of protein aggregates
purification of biomolecules separation of molecules by size |
| gptkbp:bfsParent |
gptkb:HPLC
|
| gptkbp:bfsLayer |
6
|