gel filtration chromatography
GPTKB entity
Statements (50)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
analytical technique
|
| gptkbp:alsoKnownAs |
gptkb:size_exclusion_chromatography
|
| gptkbp:analyteDoesNotInteractWith |
stationary phase chemically
|
| gptkbp:canBe |
analysis of protein complexes
buffer exchange desalting determination of quaternary structure removal of aggregates removal of salts removal of small contaminants removal of unreacted small molecules separation of nucleic acid fragments separation of oligomers separation of polysaccharide chains separation of protein aggregates separation of protein isoforms separation of synthetic polymers |
| gptkbp:commercialUse |
gptkb:Pharmacia
|
| gptkbp:commonMatrixMaterials |
dextran
agarose polyacrylamide |
| gptkbp:compatibleWith |
binding to stationary phase
|
| gptkbp:detects |
molecular weight
|
| gptkbp:developedBy |
1950s
|
| gptkbp:elutionOrder |
largest to smallest molecules
|
| gptkbp:fractionationRange |
depends on bead pore size
|
| https://www.w3.org/2000/01/rdf-schema#label |
gel filtration chromatography
|
| gptkbp:inventedBy |
gptkb:Jerker_Porath
Per Flodin |
| gptkbp:largeMolecules |
elute first
|
| gptkbp:mobilePhase |
buffer solution
|
| gptkbp:principle |
molecules separated by size
|
| gptkbp:resolution |
flow rate
bead size column length |
| gptkbp:separates |
nucleic acids
polysaccharides proteins |
| gptkbp:smallMolecules |
elute later
|
| gptkbp:stationaryPhase |
porous beads
|
| gptkbp:type |
gptkb:HPLC
gptkb:FPLC gravity flow |
| gptkbp:usedFor |
separation of molecules by size
|
| gptkbp:usedIn |
biochemistry
molecular biology protein purification polysaccharide purification |
| gptkbp:bfsParent |
gptkb:size_exclusion_HPLC
|
| gptkbp:bfsLayer |
7
|