Statements (26)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
analytical technique
|
| gptkbp:advantage |
high resolution
non-destructive separation |
| gptkbp:alternativeTo |
gptkb:SDS-PAGE
field-flow fractionation |
| gptkbp:commonDetector |
UV detector
refractive index detector multi-angle light scattering detector |
| gptkbp:fullName |
Size Exclusion Chromatography - High Performance Liquid Chromatography
|
| https://www.w3.org/2000/01/rdf-schema#label |
SEC-HPLC
|
| gptkbp:limitation |
requires calibration standards
limited separation for similar-sized molecules |
| gptkbp:mobilePhase |
aqueous buffer
organic solvent |
| gptkbp:relatedTo |
gptkb:HPLC
gptkb:gel_filtration_chromatography |
| gptkbp:separates |
molecular size
|
| gptkbp:stationaryPhase |
porous beads
|
| gptkbp:usedFor |
protein analysis
molecular weight determination aggregate detection |
| gptkbp:usedIn |
quality control
biopharmaceutical analysis monoclonal antibody characterization |
| gptkbp:bfsParent |
gptkb:size_exclusion_HPLC
|
| gptkbp:bfsLayer |
7
|