size exclusion chromatography
GPTKB entity
Statements (32)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
analytical technique
|
| gptkbp:advantage |
gentle on biomolecules
no binding to stationary phase |
| gptkbp:alsoKnownAs |
gptkb:gel_filtration_chromatography
gel permeation chromatography |
| gptkbp:application |
protein purification
polymer analysis molecular weight determination |
| gptkbp:columnMaterial |
dextran
agarose polyacrylamide |
| gptkbp:detects |
refractive index
light scattering UV absorbance |
| https://www.w3.org/2000/01/rdf-schema#label |
size exclusion chromatography
|
| gptkbp:inventedBy |
1950s
|
| gptkbp:limitation |
cannot separate molecules of similar size
|
| gptkbp:mobilePhase |
liquid solvent
|
| gptkbp:principle |
large molecules elute first
small molecules elute later |
| gptkbp:relatedTo |
gptkb:high-performance_liquid_chromatography
affinity chromatography ion exchange chromatography |
| gptkbp:requires |
calibration with standards
|
| gptkbp:separates |
molecular size
|
| gptkbp:stationaryPhase |
porous gel beads
|
| gptkbp:usedFor |
separation of molecules by size
|
| gptkbp:usedIn |
biochemistry
polymer science pharmaceutical analysis |
| gptkbp:bfsParent |
gptkb:size_exclusion_HPLC
|
| gptkbp:bfsLayer |
7
|