size exclusion high performance liquid chromatography
GPTKB entity
Statements (23)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
analytical technique
|
| gptkbp:advantage |
no interaction with stationary phase
non-destructive separation |
| gptkbp:alsoKnownAs |
gptkb:size_exclusion_chromatography
SE-HPLC |
| gptkbp:detects |
refractive index
light scattering UV absorbance |
| https://www.w3.org/2000/01/rdf-schema#label |
size exclusion high performance liquid chromatography
|
| gptkbp:limitation |
limited resolution for similar-sized molecules
not suitable for very small molecules |
| gptkbp:mobilePhase |
aqueous buffer
organic solvent |
| gptkbp:principle |
molecules separated based on hydrodynamic volume
|
| gptkbp:relatedTo |
gptkb:gel_filtration_chromatography
gel permeation chromatography |
| gptkbp:type |
porous gel column
|
| gptkbp:usedFor |
analysis of nucleic acids
analysis of polymers analysis of proteins separation of molecules by size |
| gptkbp:bfsParent |
gptkb:size_exclusion_HPLC
|
| gptkbp:bfsLayer |
7
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