enzyme-linked immunosorbent assay (ELISA)
GPTKB entity
Statements (49)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
immunoassay
|
| gptkbp:abbreviation |
gptkb:ELISA
|
| gptkbp:advantage |
high sensitivity
high specificity quantitative results relatively low cost |
| gptkbp:alternativeTo |
gptkb:immunofluorescence_assay
gptkb:lateral_flow_assay western blot |
| gptkbp:detects |
colorimetric detection
enzyme-linked antibody |
| gptkbp:developedBy |
1970s
|
| https://www.w3.org/2000/01/rdf-schema#label |
enzyme-linked immunosorbent assay (ELISA)
|
| gptkbp:inventedBy |
gptkb:Eva_Engvall
gptkb:Peter_Perlmann |
| gptkbp:limitation |
false positives
cross-reactivity requires skilled personnel |
| gptkbp:regulates |
FDA (for clinical use)
|
| gptkbp:relatedTo |
biochemistry
immunology diagnosis |
| gptkbp:replacedBy |
radioimmunoassay
|
| gptkbp:requires |
gptkb:monoclonal_antibody
immune response microtiter plate enzyme substrate enzyme conjugate |
| gptkbp:subspecies |
gptkb:competitive_ELISA
gptkb:direct_ELISA gptkb:indirect_ELISA gptkb:sandwich_ELISA |
| gptkbp:type |
gptkb:plasma
urine serum cell lysate |
| gptkbp:usedFor |
HIV testing
pregnancy testing food allergen detection hormone quantification COVID-19 antibody testing detection of antibodies detection of antigens quantification of proteins disease biomarker detection |
| gptkbp:usedIn |
research laboratories
clinical diagnostics |
| gptkbp:bfsParent |
gptkb:Enzyme-multiplied_immunoassay_technique_(EMIT)
|
| gptkbp:bfsLayer |
8
|