Statements (31)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
molecular biology technique
|
| gptkbp:advantage |
absolute quantification without standard curve
higher precision than qPCR tolerance to PCR inhibitors |
| gptkbp:application |
oncology
environmental monitoring clinical diagnostics infectious disease detection liquid biopsy GMO detection |
| gptkbp:developedBy |
gptkb:Bio-Rad_Laboratories
|
| gptkbp:firstDescribed |
2011
|
| gptkbp:fullName |
droplet digital polymerase chain reaction
|
| https://www.w3.org/2000/01/rdf-schema#label |
ddPCR
|
| gptkbp:limitation |
requires specialized equipment
higher cost than qPCR lower throughput than qPCR |
| gptkbp:principle |
Poisson statistics for quantification
end-point PCR in each droplet partitioning sample into thousands of droplets |
| gptkbp:relatedTo |
PCR
qPCR |
| gptkbp:requires |
thermal cycler
droplet generator droplet reader |
| gptkbp:usedFor |
gene expression analysis
copy number variation analysis rare mutation detection absolute quantification of nucleic acids |
| gptkbp:bfsParent |
gptkb:droplet_digital_PCR
|
| gptkbp:bfsLayer |
7
|