Statements (45)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
genome editing technology
|
| gptkbp:alternativeTo |
gptkb:CRISPR-Cas9
gptkb:TALENs meganucleases |
| gptkbp:application |
gptkb:agricultural_machinery
gene therapy functional genomics |
| gptkbp:cause |
gptkb:non-homologous_end_joining
gptkb:homology-directed_repair insertions and deletions (indels) |
| gptkbp:contrastsWith |
gptkb:TALEN_gene_editing
CRISPR gene editing |
| gptkbp:deliveryMethods |
gptkb:gene
plasmid DNA viral vectors |
| gptkbp:developedBy |
1990s
|
| gptkbp:enables |
gene knockout
gene correction gene insertion targeted DNA double-strand breaks |
| gptkbp:firstDescribed |
gptkb:Aaron_Klug
gptkb:Dana_Carroll Carlos F. Barbas III |
| gptkbp:fullName |
Zinc Finger Nuclease gene editing
|
| https://www.w3.org/2000/01/rdf-schema#label |
ZFN gene editing
|
| gptkbp:limitation |
off-target effects
complex protein design costly production |
| gptkbp:mechanismOfAction |
DNA cleavage by FokI nuclease domain
|
| gptkbp:notableCompany |
gptkb:Sangamo_Therapeutics
|
| gptkbp:patent |
gptkb:Sangamo_Therapeutics
|
| gptkbp:recognizedBy |
specific DNA sequences
|
| gptkbp:requires |
custom protein engineering
dimerization of FokI domains |
| gptkbp:usedFor |
crop improvement
animal model creation sickle cell disease research HIV gene therapy research beta-thalassemia research |
| gptkbp:usedIn |
animal genome editing
plant genome editing human cell genome editing |
| gptkbp:uses |
engineered zinc finger nucleases
|
| gptkbp:bfsParent |
gptkb:TALEN_gene_editing
|
| gptkbp:bfsLayer |
8
|