Statements (49)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
molecular biology technique
|
| gptkbp:alternativeName |
fluorescent in situ hybridization for RNA
|
| gptkbp:analyzes |
gptkb:confocal_microscopy
super-resolution microscopy fluorescence microscopy |
| gptkbp:appliesTo |
cancer research
cell biology developmental biology neuroscience |
| gptkbp:canBe |
tissue sections
cultured cells multiplexed single-molecule resolution whole-mount samples |
| gptkbp:canDistinguish |
allele-specific expression
splice variants |
| gptkbp:citation |
thousands of scientific publications
|
| gptkbp:combines |
gptkb:DNA_FISH
immunofluorescence live-cell imaging (with modifications) |
| gptkbp:commercialKitsAvailable |
gptkb:RNAscope
ViewRNA |
| gptkbp:detects |
gptkb:gene
non-coding RNA viral RNA |
| gptkbp:developedBy |
1980s
|
| gptkbp:enables |
quantification of RNA molecules
spatial mapping of RNA |
| gptkbp:fullName |
RNA fluorescence in situ hybridization
|
| https://www.w3.org/2000/01/rdf-schema#label |
RNA FISH
|
| gptkbp:improves |
branched DNA amplification
smFISH (single-molecule FISH) |
| gptkbp:limitation |
can be time-consuming
requires high-quality probes signal can be weak |
| gptkbp:relatedTo |
gptkb:DNA_FISH
in situ hybridization |
| gptkbp:requires |
microscopy
fluorescent labeling |
| gptkbp:supportsProtocol |
imaging
washing steps probe hybridization cell fixation |
| gptkbp:usedFor |
visualizing gene expression
detecting RNA molecules in cells localizing specific RNA sequences |
| gptkbp:uses |
fluorescent probes
|
| gptkbp:bfsParent |
gptkb:FISH_(fluorescence_in_situ_hybridization)
|
| gptkbp:bfsLayer |
7
|