Statements (50)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
molecular biology technique
|
| gptkbp:advantage |
multiplexing capability
applicable to interphase and metaphase cells direct visualization of DNA sequences |
| gptkbp:alternativeTo |
gptkb:Southern_blot
PCR-based detection |
| gptkbp:appliesTo |
chromosomes
cell nuclei |
| gptkbp:combines |
immunofluorescence
other cytogenetic techniques |
| gptkbp:detects |
aneuploidy
chromosomal translocations gene amplifications gene deletions |
| gptkbp:developedBy |
1980s
|
| gptkbp:enables |
single-cell analysis
spatial genome organization studies |
| gptkbp:firstDescribed |
1986
David Pinkel Joe W. Gray |
| gptkbp:fullName |
DNA Fluorescence In Situ Hybridization
|
| https://www.w3.org/2000/01/rdf-schema#label |
DNA FISH
|
| gptkbp:limitation |
can be time-consuming
may not detect small mutations requires high-quality probes resolution limited by microscopy |
| gptkbp:publishedIn |
gptkb:Proceedings_of_the_National_Academy_of_Sciences
|
| gptkbp:relatedTo |
gptkb:RNA_FISH
gptkb:chromosome_painting cytogenetics genomics |
| gptkbp:requires |
microscopy
hybridization buffer DNA denaturation fluorescently labeled DNA probes |
| gptkbp:supportsProtocol |
washing
imaging detection probe hybridization sample fixation |
| gptkbp:usedFor |
detecting specific DNA sequences
mapping gene locations visualizing chromosomal abnormalities |
| gptkbp:usedIn |
genetic research
cancer diagnostics prenatal diagnosis |
| gptkbp:uses |
fluorescent probes
|
| gptkbp:visualizes |
fluorescence microscopy
|
| gptkbp:bfsParent |
gptkb:FISH_(fluorescence_in_situ_hybridization)
|
| gptkbp:bfsLayer |
7
|