Statements (53)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
gptkb:enzyme
|
| gptkbp:cleavage_site |
variable distance from recognition site
|
| gptkbp:cuts |
outside recognition site
|
| gptkbp:example |
gptkb:FokI
BaeI BbsI BciVI BfiI BfuAI BmrI BsaI BseRI BseYI BsmAI BsmBI BspMI BspQI BsrDI BstXI BtgZI BtsI BveI Eco31I Esp3I LguI MlyI SapI SfaNI SgrAI SmiI TspMI TthHB27I Van91I XcmI ZraI |
| gptkbp:foundIn |
gptkb:Bacteria
|
| https://www.w3.org/2000/01/rdf-schema#label |
Type IIS restriction enzyme
|
| gptkbp:produces |
sticky ends
blunt ends |
| gptkbp:recognition_site |
non-palindromic
|
| gptkbp:recognizedBy |
asymmetric DNA sequences
|
| gptkbp:subspecies |
Type IIs
Type IIs/IIs Type IIs/IIs/IIs |
| gptkbp:used_in |
gptkb:Golden_Gate_Assembly
molecular cloning |
| gptkbp:usedFor |
synthetic biology
gene synthesis DNA assembly modular cloning |
| gptkbp:bfsParent |
gptkb:FokI
gptkb:FokI_endonuclease |
| gptkbp:bfsLayer |
7
|