Enzyme-Linked Immunosorbent Assay (ELISA)
GPTKB entity
Statements (50)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
immunoassay
|
| gptkbp:abbreviation |
gptkb:ELISA
|
| gptkbp:advantage |
high sensitivity
high specificity quantitative results |
| gptkbp:alternativeTo |
gptkb:lateral_flow_assay
radioimmunoassay western blot |
| gptkbp:detects |
gptkb:COVID-19
gptkb:HIV drugs cytokines autoantibodies hormones enzyme-linked detection colorimetric detection allergens hepatitis viruses bacterial toxins |
| gptkbp:developedBy |
1970s
|
| gptkbp:format |
gptkb:competitive_ELISA
gptkb:direct_ELISA gptkb:indirect_ELISA gptkb:sandwich_ELISA |
| https://www.w3.org/2000/01/rdf-schema#label |
Enzyme-Linked Immunosorbent Assay (ELISA)
|
| gptkbp:inventedBy |
gptkb:Eva_Engvall
gptkb:Peter_Perlmann |
| gptkbp:limitation |
time-consuming
cross-reactivity requires specialized equipment |
| gptkbp:method |
plate-based assay
|
| gptkbp:regulates |
FDA (for clinical use)
CLIA (for clinical use) |
| gptkbp:relatedTo |
biochemistry
immunology diagnostic tests |
| gptkbp:requires |
antibodies
substrate microtiter plate enzyme conjugates |
| gptkbp:usedFor |
diagnosis of diseases
detection of antibodies detection of antigens quantification of proteins |
| gptkbp:usedIn |
gptkb:veterinary_medicine
research laboratories food industry clinical laboratories |
| gptkbp:bfsParent |
gptkb:Eva_Engvall
|
| gptkbp:bfsLayer |
6
|