Statements (37)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
immunological assay
|
| gptkbp:advantage |
high sensitivity
single-cell resolution |
| gptkbp:alternativeName |
Enzyme-Linked ImmunoSpot assay
|
| gptkbp:commercialKitsAvailable |
gptkb:BD_Biosciences
gptkb:R&D_Systems Mabtech |
| gptkbp:detects |
gptkb:IL-2
gptkb:IFN-gamma Granzyme B antibody-secreting cells single-cell cytokine production |
| gptkbp:developedBy |
gptkb:S._Czerkinsky
1983 |
| https://www.w3.org/2000/01/rdf-schema#label |
ELISPOT assay
|
| gptkbp:limitation |
limited multiplexing
requires viable cells |
| gptkbp:measures |
number of secreting cells
|
| gptkbp:method |
cells incubated on antibody-coated plate
detection by enzyme-linked secondary antibody secreted proteins captured by antibodies visualization as spots |
| gptkbp:readBy |
automated plate reader
spot counting |
| gptkbp:relatedTo |
gptkb:ELISA
flow cytometry |
| gptkbp:type |
peripheral blood mononuclear cells
splenocytes |
| gptkbp:usedFor |
detecting cytokine secretion
measuring immune cell function enumerating antigen-specific T cells |
| gptkbp:usedIn |
vaccine development
infectious disease research cancer immunology autoimmunity research |
| gptkbp:bfsParent |
gptkb:Cytotoxic_T_Lymphocyte
|
| gptkbp:bfsLayer |
7
|