Statements (34)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
Molecular biology technique
|
| gptkbp:advantage |
High sensitivity
Absolute quantification No need for standard curves |
| gptkbp:alternativeTo |
gptkb:Quantitative_PCR
|
| gptkbp:analyzes |
gptkb:gene
|
| gptkbp:application |
Environmental monitoring
Oncology Prenatal testing Food safety testing Infectious disease diagnostics |
| gptkbp:commercialUse |
gptkb:Thermo_Fisher_Scientific
gptkb:Bio-Rad_Laboratories Stilla Technologies |
| gptkbp:detects |
Fluorescence
|
| gptkbp:developedBy |
1990s
|
| https://www.w3.org/2000/01/rdf-schema#label |
Digital PCR
|
| gptkbp:inventedBy |
Stephen A. Fodor
|
| gptkbp:limitation |
Higher cost
Requires specialized equipment Lower throughput than qPCR |
| gptkbp:partitionMethod |
gptkb:Droplet
Microfluidic chamber |
| gptkbp:principle |
End-point PCR detection
Partitioning sample into many individual reactions |
| gptkbp:publishedIn |
Vogelstein B, Kinzler KW. Digital PCR. Proc Natl Acad Sci U S A. 1999.
|
| gptkbp:relatedTo |
gptkb:Polymerase_chain_reaction
gptkb:Real-time_PCR |
| gptkbp:usedFor |
Gene expression analysis
Copy number variation analysis DNA quantification Rare mutation detection |
| gptkbp:bfsParent |
gptkb:Droplet_Digital_PCR
|
| gptkbp:bfsLayer |
8
|