Statements (50)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
Molecular biology technique
|
| gptkbp:advantage |
high sensitivity
high specificity rapid results |
| gptkbp:alsoKnownAs |
gptkb:quantitative_PCR
qPCR |
| gptkbp:canBe |
gptkb:two-step
gptkb:TaqMan_probes real-time analysis one-step absolute quantification Scorpion probes endpoint analysis relative quantification multiplexed SYBR Green dye Molecular beacons singleplexed |
| gptkbp:detects |
single nucleotide polymorphisms
|
| gptkbp:developedBy |
1990s
|
| gptkbp:enables |
quantitative measurement of RNA (via reverse transcription)
quantitative measurement of DNA |
| https://www.w3.org/2000/01/rdf-schema#label |
Real-time PCR
|
| gptkbp:inventedBy |
Kary Mullis (PCR inventor)
|
| gptkbp:limitation |
requires specialized equipment
can be affected by inhibitors requires careful experimental design |
| gptkbp:measures |
amplification of DNA in real time
|
| gptkbp:output |
amplification curve
Ct value (cycle threshold) |
| gptkbp:regulates |
MIQE guidelines
|
| gptkbp:relatedTo |
gptkb:Polymerase_chain_reaction
Reverse transcription PCR |
| gptkbp:requires |
DNA polymerase
primers thermal cycler with fluorescence detection template DNA or cDNA |
| gptkbp:usedBy |
research laboratories
clinical laboratories |
| gptkbp:usedFor |
gene expression analysis
pathogen detection quantification of nucleic acids |
| gptkbp:usedIn |
forensic science
environmental monitoring food safety testing clinical diagnostics |
| gptkbp:uses |
fluorescent dyes
sequence-specific DNA probes |
| gptkbp:bfsParent |
gptkb:Droplet_Digital_PCR
|
| gptkbp:bfsLayer |
8
|