|
gptkbp:instanceOf
|
gptkb:protein
endonuclease
|
|
gptkbp:affiliatedWith
|
gptkb:Type_V_CRISPR_system
gptkb:CRISPR-Cas_system
Class 2 CRISPR system
|
|
gptkbp:alternativeName
|
gptkb:Cas12a
|
|
gptkbp:cleavage
|
double-stranded DNA
staggered cut
|
|
gptkbp:compatibleWith
|
gptkb:tracrRNA
|
|
gptkbp:discoveredBy
|
gptkb:Feng_Zhang
|
|
gptkbp:EC_number
|
3.1.21.1
|
|
gptkbp:firstDescribed
|
2015
|
|
gptkbp:foundIn
|
gptkb:Bacteria
archaea
|
|
gptkbp:function
|
genome editing
DNA cleavage
|
|
https://www.w3.org/2000/01/rdf-schema#label
|
Cpf1 protein
|
|
gptkbp:mechanismOfAction
|
RNA-guided DNA endonuclease
|
|
gptkbp:orthologIn
|
gptkb:AsCpf1
gptkb:FnCpf1
gptkb:LbCpf1
gptkb:MbCpf1
|
|
gptkbp:PDB
|
gptkb:5B43
gptkb:5ID6
5NFV
|
|
gptkbp:produces
|
sticky ends
|
|
gptkbp:recognizesPAM
|
TTTV (where V is A, C, or G)
|
|
gptkbp:requires
|
gptkb:crRNA
|
|
gptkbp:UniProtID
|
A0A0D6JRU4
|
|
gptkbp:usedFor
|
diagnostics
base editing
prime editing
epigenome editing
gene knockout
nucleic acid detection
targeted genome modification
gene correction
gene insertion
animal genome editing
plant genome editing
transcriptional regulation
multiplex genome editing
bacterial genome editing
human genome editing
|
|
gptkbp:usedIn
|
gptkb:biotechnology
molecular biology
gene editing
genome engineering
|
|
gptkbp:bfsParent
|
gptkb:CRISPR-Cas_system
|
|
gptkbp:bfsLayer
|
7
|