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gptkbp:instanceOf
|
protein quantification assay
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|
gptkbp:advantage
|
high tolerance to detergents
stable color complex
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gptkbp:alternativeTo
|
gptkb:Lowry_assay
gptkb:Bradford_assay
|
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gptkbp:citation
|
gptkb:Smith_PK_et_al.,_Analytical_Biochemistry,_1985
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gptkbp:commerciallyAvailableFrom
|
gptkb:Bio-Rad
gptkb:Thermo_Fisher_Scientific
gptkb:Sigma-Aldrich
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gptkbp:compatibleWith
|
detergents
reducing agents
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gptkbp:detectionWavelength
|
562 nm
|
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gptkbp:developedBy
|
gptkb:Galen_T._Hermanson
gptkb:Mallia_A._Mallia
gptkb:Paul_K._Smith
gptkb:Robert_I._Krohn
1985
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gptkbp:fullName
|
gptkb:Bicinchoninic_Acid_Assay
|
|
https://www.w3.org/2000/01/rdf-schema#label
|
BCA assay
|
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gptkbp:limitation
|
interference by chelating agents
sensitive to reducing agents
|
|
gptkbp:mainReagent
|
gptkb:copper(II)_sulfate
gptkb:bicinchoninic_acid
|
|
gptkbp:principle
|
colorimetric detection of cuprous ion (Cu+) by bicinchoninic acid
|
|
gptkbp:quantificationMethod
|
spectrophotometry
|
|
gptkbp:readBy
|
purple color formation
|
|
gptkbp:sensitivity
|
0.5–40 μg/mL
|
|
gptkbp:supportsProtocol
|
incubate at 37°C for 30 minutes
measure absorbance at 562 nm
mix sample with BCA reagent
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gptkbp:type
|
gptkb:biuret_reaction
protein solution
|
|
gptkbp:usedFor
|
protein concentration determination
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gptkbp:usedIn
|
biochemistry
molecular biology
clinical diagnostics
protein purification
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gptkbp:bfsParent
|
gptkb:Lowry_protein_assay
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gptkbp:bfsLayer
|
7
|