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gptkbp:instanceOf
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protein quantification assay
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gptkbp:abbreviation
|
gptkb:BCA_assay
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gptkbp:absorbanceWavelength
|
562 nm
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gptkbp:alternativeTo
|
gptkb:Lowry_assay
gptkb:Bradford_assay
|
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gptkbp:basedOn
|
gptkb:biuret_reaction
|
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gptkbp:color
|
purple
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gptkbp:compatibleWith
|
detergents
high concentrations of reducing agents
reducing agents (at low concentrations)
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gptkbp:detects
|
copper(I) ions
|
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gptkbp:developedBy
|
gptkb:Paul_K._Smith
gptkb:Robert_I._Krohn
G. T. Hermanson
|
|
https://www.w3.org/2000/01/rdf-schema#label
|
Bicinchoninic Acid Assay
|
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gptkbp:measures
|
total protein content
|
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gptkbp:publicationYear
|
1985
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gptkbp:publishedIn
|
gptkb:Analytical_Biochemistry
|
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gptkbp:reactionTime
|
30 minutes
|
|
gptkbp:readBy
|
spectrometer
|
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gptkbp:requires
|
incubation at 37°C
|
|
gptkbp:sensitivityRange
|
20–2000 µg/mL
|
|
gptkbp:type
|
biological samples
purified protein solutions
|
|
gptkbp:usedFor
|
protein concentration determination
|
|
gptkbp:usesReagent
|
gptkb:copper(II)_sulfate
gptkb:bicinchoninic_acid
|
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gptkbp:bfsParent
|
gptkb:BCA_assay
|
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gptkbp:bfsLayer
|
8
|