Statements (25)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
biochemical assay
|
| gptkbp:alternativeTo |
gptkb:BCA_assay
gptkb:Bradford_assay |
| gptkbp:basedOn |
gptkb:Biuret_reaction
gptkb:Folin–Ciocalteu_reagent |
| gptkbp:citation |
over 300,000
|
| gptkbp:detects |
colorimetric
|
| gptkbp:developedBy |
gptkb:Oliver_H._Lowry
1951 |
| https://www.w3.org/2000/01/rdf-schema#label |
Lowry assay
|
| gptkbp:limitation |
sensitive to interfering substances
|
| gptkbp:measures |
protein concentration
|
| gptkbp:notableFor |
one of the most cited scientific papers
|
| gptkbp:publishedIn |
gptkb:Journal_of_Biological_Chemistry
|
| gptkbp:readBy |
absorbance at 750 nm
|
| gptkbp:sensitivity |
5–100 micrograms protein
|
| gptkbp:supportsProtocol |
Folin–Ciocalteu reagent added
color change measured spectrophotometrically protein reacts with copper under alkaline conditions |
| gptkbp:type |
biological samples
|
| gptkbp:usedFor |
protein quantification
|
| gptkbp:bfsParent |
gptkb:BCA_assay
gptkb:Bradford_assay gptkb:Bicinchoninic_acid_assay |
| gptkbp:bfsLayer |
8
|