Statements (26)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
biochemical assay
|
| gptkbp:absorbanceWavelength |
750 nm
|
| gptkbp:alternativeTo |
gptkb:BCA_assay
gptkb:Bradford_assay |
| gptkbp:basedOn |
gptkb:Biuret_reaction
gptkb:Folin–Ciocalteu_reagent |
| gptkbp:citation |
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. J Biol Chem. 1951;193(1):265-75.
|
| gptkbp:color |
blue
|
| gptkbp:detects |
peptide bonds
|
| gptkbp:developedBy |
gptkb:Oliver_H._Lowry
1951 |
| https://www.w3.org/2000/01/rdf-schema#label |
Lowry protein assay
|
| gptkbp:limitation |
sensitive to interfering substances
|
| gptkbp:measures |
spectrophotometry
|
| gptkbp:requires |
gptkb:Folin–Ciocalteu_reagent
alkaline copper solution |
| gptkbp:sensitivity |
5–100 micrograms protein
|
| gptkbp:supportsProtocol |
absorbance measurement
addition of Folin–Ciocalteu reagent color development incubation with copper reagent |
| gptkbp:type |
biological samples
|
| gptkbp:usedFor |
protein quantification
|
| gptkbp:bfsParent |
gptkb:Lowry
gptkb:Oliver_H._Lowry |
| gptkbp:bfsLayer |
6
|