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gptkbp:instanceOf
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protein assay
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gptkbp:absorbanceWavelength
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595 nm
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gptkbp:advantage
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gptkb:fire
simple
compatible with many buffers
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gptkbp:alternativeTo
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gptkb:Lowry_assay
gptkb:BCA_assay
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gptkbp:basedOn
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Coomassie Brilliant Blue dye binding
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gptkbp:citation
|
gptkb:Bradford,_M._M._(1976)._A_rapid_and_sensitive_method_for_the_quantitation_of_microgram_quantities_of_protein_utilizing_the_principle_of_protein-dye_binding._Analytical_Biochemistry,_72,_248-254.
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gptkbp:detects
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colorimetric
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gptkbp:developedBy
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gptkb:Marion_M._Bradford
1976
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gptkbp:dyeUsed
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gptkb:Coomassie_Brilliant_Blue_G-250
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https://www.w3.org/2000/01/rdf-schema#label
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Bradford assay
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gptkbp:limitation
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non-linear response at high protein concentrations
sensitive to detergents
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gptkbp:measures
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protein concentration
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gptkbp:sensitivity
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high
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gptkbp:supportsProtocol
|
measure absorbance at 595 nm
add dye reagent to protein sample
incubate for several minutes
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gptkbp:type
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biological samples
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gptkbp:usedFor
|
protein quantification
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gptkbp:bfsParent
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gptkb:Lowry_protein_assay
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gptkbp:bfsLayer
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7
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