Statements (50)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
molecular biology technique
|
| gptkbp:advantage |
high sensitivity
high specificity real-time quantification |
| gptkbp:alsoKnownAs |
real-time RT-PCR
|
| gptkbp:application |
agriculture
microbiology oncology research virology clinical diagnostics pathogen detection |
| gptkbp:canBe |
gptkb:TaqMan_probes
SYBR Green |
| gptkbp:contrastsWith |
conventional RT-PCR
|
| gptkbp:dataAnalysis |
absolute quantification
relative quantification |
| gptkbp:detects |
cDNA
|
| gptkbp:developedBy |
1990s
|
| gptkbp:fullName |
quantitative reverse transcription polymerase chain reaction
|
| https://www.w3.org/2000/01/rdf-schema#label |
qRT-PCR
|
| gptkbp:limitation |
requires high-quality RNA
sensitive to contamination primer-dimer formation |
| gptkbp:measures |
fluorescence intensity
initial RNA amount |
| gptkbp:output |
Ct value
amplification curve melt curve analysis |
| gptkbp:regulates |
MIQE guidelines
|
| gptkbp:relatedTo |
gptkb:conventional_PCR
gptkb:digital_PCR RT-qPCR |
| gptkbp:requires |
reverse transcriptase
DNA polymerase primers fluorescent probes template RNA |
| gptkbp:step |
reverse transcription
PCR amplification fluorescence detection |
| gptkbp:usedFor |
gene expression analysis
quantification of RNA viral load measurement |
| gptkbp:usedIn |
COVID-19 testing
HIV viral load testing cancer biomarker detection |
| gptkbp:bfsParent |
gptkb:PrimeScript_reverse_transcriptase
gptkb:Transcriptomics |
| gptkbp:bfsLayer |
8
|