Statements (30)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
molecular biology technique
|
| gptkbp:detects |
radioactive labeling
chemiluminescent labeling colorimetric labeling |
| gptkbp:field |
gptkb:biotechnology
molecular genetics |
| https://www.w3.org/2000/01/rdf-schema#label |
Southern blot
|
| gptkbp:introducedIn |
1975
|
| gptkbp:inventedBy |
Edwin Southern
|
| gptkbp:involves |
gel electrophoresis
hybridization with labeled probe DNA transfer to membrane |
| gptkbp:membraneType |
gptkb:nylon
nitrocellulose |
| gptkbp:namedAfter |
Edwin Southern
|
| gptkbp:relatedTo |
gptkb:Northern_blot
gptkb:Western_blot |
| gptkbp:step |
hybridization
detection denaturation electrophoresis restriction enzyme digestion blotting |
| gptkbp:type |
gptkb:gene
|
| gptkbp:usedFor |
detection of specific DNA sequences
|
| gptkbp:usedIn |
gptkb:genetic_fingerprinting
gene mapping diagnosis of genetic disorders |
| gptkbp:bfsParent |
gptkb:Western_blot
|
| gptkbp:bfsLayer |
6
|