|
gptkbp:instanceOf
|
analytical technique
|
|
gptkbp:alternativeTo
|
isoelectric focusing
native PAGE
|
|
gptkbp:analyzes
|
protein purity
protein size
|
|
gptkbp:canBe
|
protein identification
protein quantification
protein-protein interaction studies
molecular weight estimation
monitoring protein expression
monitoring protein purification
protein fingerprinting
purity assessment
|
|
gptkbp:canBeAnalytical
|
true
|
|
gptkbp:canBePreparative
|
true
|
|
gptkbp:canBeQuantitative
|
true
|
|
gptkbp:commonBufferSystem
|
gptkb:Tris-Tricine
gptkb:Tris-glycine
|
|
gptkbp:denaturesProteins
|
true
|
|
gptkbp:developedBy
|
gptkb:Ulrich_K._Laemmli
|
|
gptkbp:firstDescribed
|
1970
|
|
gptkbp:followedBy
|
gptkb:Western_blot
protein transfer
|
|
gptkbp:fullName
|
gptkb:Sodium_dodecyl_sulfate_polyacrylamide_gel_electrophoresis
|
|
gptkbp:gelType
|
resolving gel
stacking gel
|
|
gptkbp:givesProteinsNegativeCharge
|
true
|
|
https://www.w3.org/2000/01/rdf-schema#label
|
SDS-PAGE
|
|
gptkbp:migrationDirection
|
from cathode to anode
|
|
gptkbp:proteinMigrationDependsOn
|
molecular weight
|
|
gptkbp:proteinMigrationIndependentOf
|
protein charge
protein shape
|
|
gptkbp:reduces
|
beta-mercaptoethanol
dithiothreitol
|
|
gptkbp:requires
|
gptkb:battery
electrophoresis apparatus
buffer system
|
|
gptkbp:samplePreparationIncludes
|
boiling with SDS and reducing agent
|
|
gptkbp:separates
|
molecular weight
|
|
gptkbp:usedFor
|
separating proteins by molecular weight
|
|
gptkbp:usedIn
|
biochemistry
molecular biology
proteomics
|
|
gptkbp:usesReagent
|
polyacrylamide gel
sodium dodecyl sulfate
|
|
gptkbp:visualizes
|
gptkb:Coomassie_Brilliant_Blue_stain
silver stain
|
|
gptkbp:bfsParent
|
gptkb:Western_blot
|
|
gptkbp:bfsLayer
|
6
|