Statements (28)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
molecular biology technique
|
| gptkbp:advantage |
high efficiency
modular cloning simultaneous assembly of multiple DNA fragments |
| gptkbp:alternativeTo |
traditional restriction-ligation cloning
|
| gptkbp:canAssemble |
multiple DNA fragments in defined order
|
| gptkbp:developedBy |
gptkb:Tom_Ellis
2008 Marcos Bibb |
| gptkbp:enables |
scarless DNA assembly
one-pot DNA assembly |
| https://www.w3.org/2000/01/rdf-schema#label |
Golden Gate Assembly
|
| gptkbp:publishedIn |
gptkb:Nature_Methods
|
| gptkbp:relatedTo |
Gibson Assembly
GoldenBraid MoClo |
| gptkbp:requires |
thermocycler
unique overhangs |
| gptkbp:supportsProtocol |
digestion and ligation in single reaction
|
| gptkbp:usedBy |
research laboratories worldwide
|
| gptkbp:usedFor |
DNA assembly
|
| gptkbp:usedIn |
cloning
synthetic biology genetic engineering |
| gptkbp:uses |
T4 DNA ligase
type IIS restriction enzymes |
| gptkbp:bfsParent |
gptkb:Fenton_Assembly
|
| gptkbp:bfsLayer |
6
|