TALENs (Transcription Activator-Like Effector Nucleases)

GPTKB entity

Predicate	Object
gptkbp:instanceOf	gptkb:artificial_restriction_enzyme gptkb:genome_editing_technology
gptkbp:advantage	high specificity
gptkbp:application	disease modeling functional genomics gene therapy research
gptkbp:component	gptkb:FokI_endonuclease transcription activator-like effector (TALE) proteins
gptkbp:composedOf	FokI nuclease domain DNA-binding domain
gptkbp:developedBy	gptkb:Joung_Lab gptkb:Voytas_Lab
gptkbp:firstDescribed	2010
gptkbp:limitation	delivery challenges labor-intensive design
gptkbp:mechanismOfAction	induces double-strand breaks in DNA
gptkbp:patent	gptkb:Cellectis gptkb:Sangamo_BioSciences
gptkbp:recognitionCode	TALE repeats recognize single DNA bases
gptkbp:relatedTo	gptkb:CRISPR-Cas9 gptkb:Zinc_finger_nucleases
gptkbp:targetRecognition	customizable DNA sequence
gptkbp:usedFor	genome editing gene knockout gene insertion gene modification
gptkbp:usedIn	animal genome editing plant genome editing human cell genome editing
gptkbp:bfsParent	gptkb:TALE_proteins
gptkbp:bfsLayer	8
https://www.w3.org/2000/01/rdf-schema#label	TALENs (Transcription Activator-Like Effector Nucleases)