Biuret reaction

GPTKB entity

Statements (23)
Predicate Object
gptkbp:instanceOf gptkb:chemical_compound
gptkbp:color blue to violet
presence of proteins
gptkbp:compatibleWith short peptides
free amino acids
gptkbp:discoveredBy gptkb:Gustav_Piotrowski
gptkbp:discoveredIn 1857
https://www.w3.org/2000/01/rdf-schema#label Biuret reaction
gptkbp:mechanismOfAction complex formation between copper ions and peptide bonds
gptkbp:namedAfter gptkb:biuret_compound
gptkbp:reactant gptkb:biuret_reagent
gptkbp:reagentContains gptkb:copper(II)_sulfate
potassium iodide
sodium hydroxide
potassium sodium tartrate
gptkbp:requires alkaline conditions
gptkbp:sensitivity requires at least two peptide bonds
gptkbp:usedFor protein quantification
detecting peptide bonds
gptkbp:usedIn clinical chemistry
biochemistry laboratories
gptkbp:bfsParent gptkb:Lowry_protein_assay
gptkbp:bfsLayer 7