Enzyme-linked immunospot (ELISPOT)
GPTKB entity
Statements (34)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
immunological assay
|
| gptkbp:abbreviation |
gptkb:ELISPOT
|
| gptkbp:alternativeTo |
flow cytometry
intracellular cytokine staining |
| gptkbp:analyte |
cytokines
antibodies |
| gptkbp:application |
vaccine development
infectious disease research cancer immunology autoimmune disease research |
| gptkbp:detects |
enzyme-linked antibody
|
| gptkbp:developedBy |
gptkb:S._Czerkinsky
1983 |
| https://www.w3.org/2000/01/rdf-schema#label |
Enzyme-linked immunospot (ELISPOT)
|
| gptkbp:limitation |
labor-intensive
limited multiplexing compared to flow cytometry subjective spot counting |
| gptkbp:multiplexing |
possible
|
| gptkbp:quantitative |
yes
|
| gptkbp:readBy |
spot formation
|
| gptkbp:readoutDevice |
ELISPOT reader
|
| gptkbp:relatedTo |
gptkb:ELISA
|
| gptkbp:requires |
capture antibodies
cell culture plates detection antibodies substrate for enzyme reaction |
| gptkbp:sensitivity |
high
|
| gptkbp:type |
peripheral blood mononuclear cells
|
| gptkbp:usedFor |
detecting cytokine secretion
measuring immune cell function quantifying antigen-specific B cells quantifying antigen-specific T cells |
| gptkbp:bfsParent |
gptkb:Enzyme_Immunoassay
|
| gptkbp:bfsLayer |
7
|