Statements (53)
Predicate | Object |
---|---|
gptkbp:instanceOf |
immunoassay
Analytical technique |
gptkbp:advantage |
High sensitivity
High specificity No radioactive materials |
gptkbp:alternativeTo |
gptkb:Radioimmunoassay
|
gptkbp:canBe |
Quantitative
Qualitative |
gptkbp:detects |
gptkb:poisoning
Pathogens Allergens Drugs Color change Proteins Small molecules Hormones Fluorescence Antibodies to infectious agents Bacterial antigens Cytokines Luminescence Peptides Viral antigens |
gptkbp:developedBy |
1970s
|
https://www.w3.org/2000/01/rdf-schema#label |
Enzyme Immunoassay
|
gptkbp:relatedTo |
Biochemistry
Immunology Clinical chemistry |
gptkbp:requires |
Incubation steps
Microplate reader Washing steps |
gptkbp:step |
Addition of enzyme-labeled reagent
Addition of substrate Binding of antigen or antibody Detection of signal |
gptkbp:subspecies |
gptkb:ELISA
gptkb:Enzyme-linked_immunosorbent_assay_(ELISA) gptkb:Enzyme-linked_immunospot_(ELISPOT) gptkb:Enzyme-multiplied_immunoassay_technique_(EMIT) |
gptkbp:usedFor |
Clinical diagnostics
Detection of antibodies Detection of antigens Quantification of proteins |
gptkbp:usedIn |
gptkb:Food_industry
gptkb:Medical_laboratories Pharmaceutical industry Environmental testing |
gptkbp:uses |
Antibody
Antigen Enzyme-labeled reagents Substrate for enzyme |
gptkbp:bfsParent |
gptkb:EI
|
gptkbp:bfsLayer |
6
|