Statements (27)
Predicate | Object |
---|---|
gptkbp:instanceOf |
immunological assay
|
gptkbp:advantage |
high sensitivity
single-cell resolution |
gptkbp:analyte |
cytokines
antibodies |
gptkbp:application |
vaccine development
infectious disease research cancer immunology autoimmunity research |
gptkbp:detects |
enzyme-linked immunosorbent
|
gptkbp:developedBy |
gptkb:S._Czerkinsky
1983 |
gptkbp:fullName |
Enzyme-Linked ImmunoSpot
|
https://www.w3.org/2000/01/rdf-schema#label |
ELISPOT
|
gptkbp:limitation |
labor-intensive
limited multiplexing |
gptkbp:method |
cellular assay
|
gptkbp:readBy |
spot formation
|
gptkbp:relatedTo |
gptkb:ELISA
flow cytometry |
gptkbp:type |
peripheral blood mononuclear cells
|
gptkbp:usedFor |
detection of cytokine secretion
enumeration of antigen-specific B cells enumeration of antigen-specific T cells measurement of immune cell function |
gptkbp:bfsParent |
gptkb:IFN-γ
|
gptkbp:bfsLayer |
7
|