ELISPOT

GPTKB entity

Statements (27)
Predicate Object
gptkbp:instanceOf immunological assay
gptkbp:advantage high sensitivity
single-cell resolution
gptkbp:analyte cytokines
antibodies
gptkbp:application vaccine development
infectious disease research
cancer immunology
autoimmunity research
gptkbp:detects enzyme-linked immunosorbent
gptkbp:developedBy gptkb:S._Czerkinsky
1983
gptkbp:fullName Enzyme-Linked ImmunoSpot
https://www.w3.org/2000/01/rdf-schema#label ELISPOT
gptkbp:limitation labor-intensive
limited multiplexing
gptkbp:method cellular assay
gptkbp:readBy spot formation
gptkbp:relatedTo gptkb:ELISA
flow cytometry
gptkbp:type peripheral blood mononuclear cells
gptkbp:usedFor detection of cytokine secretion
enumeration of antigen-specific B cells
enumeration of antigen-specific T cells
measurement of immune cell function
gptkbp:bfsParent gptkb:IFN-γ
gptkbp:bfsLayer 7