Enhanced Green Fluorescent Protein

URI: https://gptkb.org/entity/Enhanced_Green_Fluorescent_Protein
GPTKB entity
Statements (33)
Predicate Object
gptkbp:instanceOf gptkb:protein
gptkbp:abbreviation gptkb:EGFP
gptkbp:category fluorescent proteins
gptkbp:chromophore Ser65-Tyr66-Gly67
gptkbp:derivedFrom gptkb:Aequorea_victoria_green_fluorescent_protein
gptkbp:discoveredIn 1995
gptkbp:emitsFluorescenceAt 509 nm
gptkbp:excitationPeak 488 nm
gptkbp:expressedIn gptkb:yeast
gptkb:Bacteria
plants
mammalian cells
https://www.w3.org/2000/01/rdf-schema#label Enhanced Green Fluorescent Protein
gptkbp:improves brighter fluorescence
faster chromophore maturation
greater photostability
gptkbp:molecularWeight ~27 kDa
gptkbp:mutationAssociatedWith gptkb:green_fluorescent_protein
gptkb:F64L
gptkb:S65T
gptkbp:PDB 2Y0G
gptkbp:relatedTo gptkb:Yellow_Fluorescent_Protein
gptkb:Blue_Fluorescent_Protein
gptkb:Cyan_Fluorescent_Protein
gptkbp:usedAs reporter gene
gptkbp:usedFor live cell imaging
gene expression monitoring
protein localization studies
gptkbp:usedIn cell biology
molecular biology
fluorescence microscopy
gptkbp:bfsParent gptkb:EGFP
gptkbp:bfsLayer 6