Enhanced Green Fluorescent Protein

GPTKB entity

Predicate	Object
gptkbp:instanceOf	gptkb:protein
gptkbp:abbreviation	gptkb:EGFP
gptkbp:category	fluorescent proteins
gptkbp:chromophore	Ser65-Tyr66-Gly67
gptkbp:derivedFrom	gptkb:Aequorea_victoria_green_fluorescent_protein
gptkbp:discoveredIn	1995
gptkbp:emitsFluorescenceAt	509 nm
gptkbp:excitationPeak	488 nm
gptkbp:expressedIn	gptkb:yeast gptkb:Bacteria plants mammalian cells
gptkbp:improves	brighter fluorescence faster chromophore maturation greater photostability
gptkbp:molecularWeight	~27 kDa
gptkbp:mutationAssociatedWith	gptkb:green_fluorescent_protein gptkb:F64L gptkb:S65T
gptkbp:PDB	2Y0G
gptkbp:relatedTo	gptkb:Yellow_Fluorescent_Protein gptkb:Blue_Fluorescent_Protein gptkb:Cyan_Fluorescent_Protein
gptkbp:usedAs	reporter gene
gptkbp:usedFor	live cell imaging gene expression monitoring protein localization studies
gptkbp:usedIn	cell biology molecular biology fluorescence microscopy
gptkbp:bfsParent	gptkb:EGFP
gptkbp:bfsLayer	6
https://www.w3.org/2000/01/rdf-schema#label	Enhanced Green Fluorescent Protein