Statements (53)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
Microscopy technique
|
| gptkbp:abbreviation |
Cryo-EM
|
| gptkbp:advantage |
no need for crystallization
minimal sample damage native state imaging |
| gptkbp:alternativeTo |
X-ray crystallography
NMR spectroscopy |
| gptkbp:field |
biochemistry
molecular biology structural biology |
| gptkbp:firstDemonstrated |
1980s
|
| https://www.w3.org/2000/01/rdf-schema#label |
Cryo-Electron Microscopy
|
| gptkbp:imagingMode |
gptkb:electron_tomography
single-particle analysis cryo-electron crystallography |
| gptkbp:inventedBy |
gptkb:Jacques_Dubochet
gptkb:Joachim_Frank gptkb:Richard_Henderson |
| gptkbp:limitation |
radiation damage
complex data processing sample thickness |
| gptkbp:NobelPrizeYear |
2017 Nobel Prize in Chemistry
|
| gptkbp:notableBuilding |
ion channels
ribosome membrane proteins |
| gptkbp:output |
2D images
3D reconstructions |
| gptkbp:relatedTo |
gptkb:Transmission_Electron_Microscopy
Cryo-electron crystallography Electron tomography Single-particle analysis |
| gptkbp:requires |
high vacuum
low electron dose |
| gptkbp:resolution |
near-atomic
|
| gptkbp:samplePreparation |
vitrification
|
| gptkbp:sampleState |
frozen-hydrated
|
| gptkbp:software |
CryoSPARC
EMAN2 RELION cisTEM |
| gptkbp:type |
cells
proteins viruses organelles macromolecular complexes |
| gptkbp:usedFor |
structural biology
protein structure determination virus structure analysis macromolecular complex imaging |
| gptkbp:uses |
gptkb:electron_microscope
cryogenic temperatures |
| gptkbp:bfsParent |
gptkb:TEM
|
| gptkbp:bfsLayer |
6
|