Statements (16)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
engineered protein
|
| gptkbp:alternativeTo |
wild-type Cas9
|
| gptkbp:component |
gptkb:CRISPR-Cas_system
|
| gptkbp:derivedFrom |
gptkb:Streptococcus_pyogenes_Cas9
|
| gptkbp:function |
induces single-strand DNA breaks
|
| https://www.w3.org/2000/01/rdf-schema#label |
Cas9 nickase
|
| gptkbp:mutationAssociatedWith |
D10A
H840A |
| gptkbp:reduces |
off-target effects
|
| gptkbp:requires |
gptkb:guide_RNA
|
| gptkbp:usedFor |
gptkb:homology-directed_repair
paired nicking strategy |
| gptkbp:usedIn |
genome editing
base editing |
| gptkbp:bfsParent |
gptkb:BE3
|
| gptkbp:bfsLayer |
8
|