Statements (52)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
biological technique
sequencing method |
| gptkbp:alternativeName |
single-cell transcriptomics
scRNA-seq |
| gptkbp:analyzes |
RNA molecules
single cells |
| gptkbp:developedBy |
2009
|
| gptkbp:enables |
cancer research
disease research spatial transcriptomics differential expression analysis multi-omics integration developmental biology studies immune profiling cell lineage tracing analysis of gene expression variability discovery of new cell types study of rare cell populations trajectory inference |
| gptkbp:field |
genomics
molecular biology bioinformatics systems biology |
| gptkbp:firstPublished |
gptkb:Nature_Methods
2009 Tang et al. |
| https://www.w3.org/2000/01/rdf-schema#label |
single-cell RNA-seq
|
| gptkbp:limitation |
high cost
complex data analysis dropout events technical noise |
| gptkbp:relatedTo |
single-cell genomics
RNA sequencing |
| gptkbp:requires |
reverse transcription
next-generation sequencing amplification library preparation cell isolation |
| gptkbp:supportsProtocol |
10x Genomics Chromium
CEL-Seq Drop-seq MARS-seq Smart-seq |
| gptkbp:type |
transcriptomic data
|
| gptkbp:usedFor |
gene expression profiling
transcriptome analysis cellular heterogeneity analysis identification of cell types |
| gptkbp:bfsParent |
gptkb:RNA-seq
gptkb:LINCS gptkb:Single_Cell_Type_Atlas |
| gptkbp:bfsLayer |
7
|