Statements (29)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
RNA molecule
|
| gptkbp:binds |
gptkb:Cas9_protein
target DNA |
| gptkbp:composedOf |
gptkb:crRNA
gptkb:tracrRNA |
| gptkbp:discoveredIn |
2012
|
| gptkbp:enables |
genome editing
|
| gptkbp:fullName |
single-guide RNA
|
| gptkbp:function |
guides Cas9 to target DNA sequence
|
| https://www.w3.org/2000/01/rdf-schema#label |
sgRNA
|
| gptkbp:length |
~100 nucleotides
|
| gptkbp:origin |
synthetic fusion of crRNA and tracrRNA
|
| gptkbp:requires |
PAM sequence in target DNA
|
| gptkbp:sequenceSpecificity |
determined by spacer region
|
| gptkbp:spacerRegionLength |
~20 nucleotides
|
| gptkbp:targetRecognition |
complementary base pairing
|
| gptkbp:usedFor |
base editing
epigenome editing gene activation gene knockout gene repression |
| gptkbp:usedIn |
gptkb:agricultural_machinery
gptkb:CRISPR-Cas9_system biomedical research gene therapy research bacterial adaptive immunity |
| gptkbp:bfsParent |
gptkb:CRISPR-Cas_system
gptkb:crRNA |
| gptkbp:bfsLayer |
7
|