Statements (50)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
gptkb:enzyme
|
| gptkbp:alsoKnownAs |
restriction enzymes
|
| gptkbp:application |
genetic engineering
genome editing molecular cloning DNA mapping |
| gptkbp:cofactor |
Mg2+
|
| gptkbp:discoveredBy |
gptkb:Daniel_Nathans
gptkb:Werner_Arber gptkb:Hamilton_O._Smith |
| gptkbp:example |
BamHI
EcoRI HindIII NotI PstI SmaI XhoI |
| gptkbp:firstDescribed |
1970
|
| gptkbp:foundIn |
gptkb:Bacteria
archaea |
| gptkbp:function |
cleave DNA at specific sequences
|
| gptkbp:hasBiologicalActivity |
defense against bacteriophages
|
| https://www.w3.org/2000/01/rdf-schema#label |
restriction endonucleases
|
| gptkbp:mechanismOfAction |
hydrolysis of phosphodiester bond
|
| gptkbp:NobelPrizeYear |
1978 Nobel Prize in Physiology or Medicine
|
| gptkbp:product |
DNA fragments with blunt ends
DNA fragments with sticky ends |
| gptkbp:recognitionSite |
specific DNA sequence
|
| gptkbp:type |
Type I restriction enzyme
Type II restriction enzyme Type III restriction enzyme Type IV restriction enzyme |
| gptkbp:usedIn |
synthetic biology
gene therapy site-directed mutagenesis genetic mapping recombinant DNA technology DNA fingerprinting transgenic organism creation genome mapping diagnostic assays gene cloning DNA sequencing preparation DNA library construction plasmid construction vector design restriction fragment length polymorphism (RFLP) analysis |
| gptkbp:bfsParent |
gptkb:PD-(D/E)XK_nuclease_superfamily
gptkb:PD-(D/E)XK_nuclease_superfamily_profile |
| gptkbp:bfsLayer |
7
|