Statements (47)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
Laboratory Technique
|
| gptkbp:alternativeName |
Immunoblot
|
| gptkbp:analyte |
Proteins
|
| gptkbp:application |
gptkb:HIV_Testing
Research Disease Diagnosis Protein Expression Analysis Verification of Protein Purification |
| gptkbp:commonMembranes |
gptkb:PVDF
Nitrocellulose |
| gptkbp:detectionMethods |
Fluorescence
Chemiluminescence Colorimetric Detection |
| gptkbp:developedBy |
gptkb:W._Neal_Burnette
1979 |
| gptkbp:enzyme |
Alkaline Phosphatase
Horseradish Peroxidase |
| gptkbp:firstPublished |
gptkb:Analytical_Biochemistry
1981 |
| https://www.w3.org/2000/01/rdf-schema#label |
Western Blot
|
| gptkbp:limitation |
Low Throughput
Requires Specific Antibodies Time Consuming |
| gptkbp:primaryAntibody |
Protein-Specific Antibody
|
| gptkbp:quantitative |
Semi-Quantitative
|
| gptkbp:relatedTo |
gptkb:ELISA
gptkb:SDS-PAGE Dot Blot Northern Blot Southern Blot |
| gptkbp:secondaryAntibody |
Enzyme-Conjugated Antibody
|
| gptkbp:step |
Gel Electrophoresis
Sample Preparation Blocking Detection Antibody Incubation Transfer to Membrane |
| gptkbp:type |
Cell Lysate
Tissue Lysate |
| gptkbp:usedFor |
Protein Identification
Protein Detection Protein Quantification |
| gptkbp:uses |
Gel Electrophoresis
Antibody Probing Membrane Transfer |
| gptkbp:bfsParent |
gptkb:Antikörper
|
| gptkbp:bfsLayer |
7
|