Statements (33)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
DNA sequencing method
|
| gptkbp:advantage |
high accuracy
long read length compared to NGS |
| gptkbp:alsoKnownAs |
chain-termination method
|
| gptkbp:application |
genome sequencing
genetic research mutation detection |
| gptkbp:category |
Genetics
Molecular biology technique |
| gptkbp:detects |
gel electrophoresis
capillary electrophoresis |
| gptkbp:enables |
determination of nucleotide sequence
|
| gptkbp:firstPublished |
gptkb:Proceedings_of_the_National_Academy_of_Sciences
1977 |
| https://www.w3.org/2000/01/rdf-schema#label |
Sanger sequencing method
|
| gptkbp:introducedIn |
1977
|
| gptkbp:inventedBy |
gptkb:Frederick_Sanger
|
| gptkbp:limitation |
low throughput
expensive for large-scale projects labor intensive read length up to 1000 bases |
| gptkbp:NobelPrizeYear |
Frederick Sanger won Nobel Prize in Chemistry
|
| gptkbp:relatedTo |
gptkb:Maxam-Gilbert_sequencing
|
| gptkbp:replacedBy |
next-generation sequencing
|
| gptkbp:significance |
first widely used DNA sequencing method
|
| gptkbp:usedFor |
DNA sequencing
|
| gptkbp:uses |
DNA polymerase
primers deoxynucleotides dideoxynucleotides template DNA |
| gptkbp:bfsParent |
gptkb:Maxam-Gilbert_sequencing_method
|
| gptkbp:bfsLayer |
7
|