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gptkbp:instanceOf
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gptkb:DNA_sequencing_method
|
|
gptkbp:advantage
|
high accuracy
long read length compared to NGS
|
|
gptkbp:alsoKnownAs
|
chain-termination method
|
|
gptkbp:application
|
genome sequencing
genetic research
mutation detection
|
|
gptkbp:category
|
gptkb:Molecular_biology_technique
Genetics
|
|
gptkbp:detects
|
gel electrophoresis
capillary electrophoresis
|
|
gptkbp:enables
|
determination of nucleotide sequence
|
|
gptkbp:firstPublished
|
gptkb:Proceedings_of_the_National_Academy_of_Sciences
1977
|
|
gptkbp:introducedIn
|
1977
|
|
gptkbp:inventedBy
|
gptkb:Frederick_Sanger
|
|
gptkbp:limitation
|
low throughput
expensive for large-scale projects
labor intensive
read length up to 1000 bases
|
|
gptkbp:NobelPrizeYear
|
Frederick Sanger won Nobel Prize in Chemistry
|
|
gptkbp:relatedTo
|
gptkb:Maxam-Gilbert_sequencing
|
|
gptkbp:replacedBy
|
next-generation sequencing
|
|
gptkbp:significance
|
first widely used DNA sequencing method
|
|
gptkbp:usedFor
|
DNA sequencing
|
|
gptkbp:uses
|
gptkb:DNA_polymerase
primers
deoxynucleotides
dideoxynucleotides
template DNA
|
|
gptkbp:bfsParent
|
gptkb:Maxam-Gilbert_sequencing_method
|
|
gptkbp:bfsLayer
|
7
|
|
https://www.w3.org/2000/01/rdf-schema#label
|
Sanger sequencing method
|