Statements (23)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
DNA sequencing method
|
| gptkbp:alsoKnownAs |
chemical sequencing
|
| gptkbp:developedBy |
gptkb:Walter_Gilbert
gptkb:Allan_Maxam |
| gptkbp:historicalSignificance |
first widely used DNA sequencing method
|
| https://www.w3.org/2000/01/rdf-schema#label |
Maxam-Gilbert sequencing
|
| gptkbp:introducedIn |
1977
|
| gptkbp:limitation |
complex protocol
difficult automation use of hazardous chemicals |
| gptkbp:method |
chemical cleavage method
|
| gptkbp:notableFor |
sequencing of bacteriophage phiX174 DNA
|
| gptkbp:publicationYear |
1977
|
| gptkbp:publishedIn |
gptkb:Proceedings_of_the_National_Academy_of_Sciences
|
| gptkbp:replacedBy |
gptkb:Sanger_sequencing
|
| gptkbp:requires |
radioactive labeling
toxic chemicals |
| gptkbp:step |
gel electrophoresis
chemical modification of DNA cleavage at specific bases |
| gptkbp:usedFor |
DNA sequencing
|
| gptkbp:bfsParent |
gptkb:Walter_Gilbert
|
| gptkbp:bfsLayer |
6
|