Maxam-Gilbert sequencing method
GPTKB entity
Statements (20)
Predicate | Object |
---|---|
gptkbp:instanceOf |
DNA sequencing method
|
gptkbp:alsoKnownAs |
chemical sequencing
|
gptkbp:developedBy |
gptkb:Walter_Gilbert
gptkb:Allan_Maxam 1977 |
gptkbp:historicalSignificance |
first widely used DNA sequencing method
|
https://www.w3.org/2000/01/rdf-schema#label |
Maxam-Gilbert sequencing method
|
gptkbp:limitation |
complex protocol
difficult automation use of hazardous chemicals |
gptkbp:method |
chemical cleavage
|
gptkbp:notablePublication |
gptkb:A_new_method_for_sequencing_DNA_(PNAS,_1977)
|
gptkbp:replacedBy |
gptkb:Sanger_sequencing_method
chain-termination method |
gptkbp:requires |
radioactive labeling
chemical modification of DNA polyacrylamide gel electrophoresis |
gptkbp:usedFor |
DNA sequencing
|
gptkbp:bfsParent |
gptkb:Walter_Gilbert
|
gptkbp:bfsLayer |
6
|