Maxam-Gilbert sequencing method

GPTKB entity

Statements (20)
Predicate Object
gptkbp:instanceOf DNA sequencing method
gptkbp:alsoKnownAs chemical sequencing
gptkbp:developedBy gptkb:Walter_Gilbert
gptkb:Allan_Maxam
1977
gptkbp:historicalSignificance first widely used DNA sequencing method
https://www.w3.org/2000/01/rdf-schema#label Maxam-Gilbert sequencing method
gptkbp:limitation complex protocol
difficult automation
use of hazardous chemicals
gptkbp:method chemical cleavage
gptkbp:notablePublication gptkb:A_new_method_for_sequencing_DNA_(PNAS,_1977)
gptkbp:replacedBy gptkb:Sanger_sequencing_method
chain-termination method
gptkbp:requires radioactive labeling
chemical modification of DNA
polyacrylamide gel electrophoresis
gptkbp:usedFor DNA sequencing
gptkbp:bfsParent gptkb:Walter_Gilbert
gptkbp:bfsLayer 6