Coomassie Brilliant Blue stain
GPTKB entity
Statements (22)
Predicate | Object |
---|---|
gptkbp:instanceOf |
biological stain
|
gptkbp:alternativeTo |
silver stain
|
gptkbp:application |
biochemistry
molecular biology |
gptkbp:bindingMechanism |
binds to basic and aromatic amino acids
|
gptkbp:color |
blue
|
gptkbp:component |
gptkb:Coomassie_Brilliant_Blue_G-250
Coomassie Brilliant Blue R-250 |
gptkbp:detects |
proteins
|
gptkbp:discoveredIn |
1960s
|
https://www.w3.org/2000/01/rdf-schema#label |
Coomassie Brilliant Blue stain
|
gptkbp:quantificationMethod |
gptkb:Bradford_assay
|
gptkbp:removes |
destaining with methanol and acetic acid
|
gptkbp:riskFactor |
irritant
|
gptkbp:solubility |
soluble in methanol and acetic acid
|
gptkbp:storage |
room temperature
|
gptkbp:usedFor |
protein staining
|
gptkbp:usedIn |
gptkb:SDS-PAGE
polyacrylamide gel electrophoresis |
gptkbp:visualizationMethod |
visible by eye
|
gptkbp:bfsParent |
gptkb:SDS-PAGE
|
gptkbp:bfsLayer |
7
|