Statements (44)
| Predicate | Object |
|---|---|
| gptkbp:instanceOf |
DNA sequencing method
|
| gptkbp:advantage |
does not require prior knowledge of gene sequences
|
| gptkbp:alternativeTo |
microarray analysis
EST sequencing |
| gptkbp:analyzes |
gptkb:gene
|
| gptkbp:citation |
high
|
| gptkbp:contrastsWith |
microarray
|
| gptkbp:developedBy |
gptkb:Bert_Vogelstein
gptkb:Kenneth_W._Kinzler V. E. Velculescu Victor E. Velculescu |
| gptkbp:enables |
quantitative analysis of gene expression
|
| gptkbp:fullName |
Serial Analysis of Gene Expression sequencing
|
| gptkbp:generation |
short sequence tags
|
| https://www.w3.org/2000/01/rdf-schema#label |
SAGE sequencing
|
| gptkbp:impact |
enabled discovery of novel transcripts
quantitative transcriptome analysis |
| gptkbp:limitation |
labor-intensive
requires large amounts of RNA short tag length may cause ambiguity |
| gptkbp:method |
high-throughput sequencing
|
| gptkbp:publicationYear |
1995
|
| gptkbp:publishedIn |
gptkb:science
|
| gptkbp:relatedTo |
gptkb:CAGE_sequencing
RNA-Seq |
| gptkbp:step |
cloning
sequencing concatenation PCR amplification restriction enzyme digestion cDNA synthesis linker ligation |
| gptkbp:tagLength |
10-14 base pairs
|
| gptkbp:type |
digital gene expression data
|
| gptkbp:usedBy |
genomics researchers
molecular biologists |
| gptkbp:usedFor |
gene expression profiling
|
| gptkbp:usedIn |
functional genomics
transcriptomics |
| gptkbp:variant |
DeepSAGE
LongSAGE SuperSAGE |
| gptkbp:bfsParent |
gptkb:CAGE_sequencing
|
| gptkbp:bfsLayer |
7
|